Enhancing two-photon microscopy with the VINCI-1064 femtosecond Laser
On: Dec 20, 2024
In: Ultrafast Lasers
In two-photon microscopy, green fluorophores excited with 920 nm femtosecond lasers have been the standard choice for researchers. However, there is an increased interest in the use of red fluorophores such as mCherry or tdTomato that requires excitation at longer wavelengths near 1064 nm. At the same time, the ability to combine 920 nm and 1064 nm light sources is becoming more popular, paving the way for advanced multicolour imaging techniques.
Longer wavelengths like 1064 nm offer significant advantages such as deeper tissue penetration, reduced scattering and photo-damage, and less background auto-fluorescence compared to light in the 700 to 900 nm window. Despite these benefits, many Ti:Sa lasers are limited in their ability to provide sufficient power at longer wavelengths, which can frustrate researchers looking to fully harness the potential of red fluorophores in their experiments.
To address these challenges, TeraXion has developed the VINCI-1064 femtosecond laser, a powerful yet affordable solution designed to bring 1064 nm capability to two-photon microscopy systems. This state-of-the-art laser features exceptionally high peak power—up to 1 MW—delivering a significant increase in fluorescence intensity compared to Ti:Sa lasers or other fixed wavelength lasers on the market. Its design ensures seamless integration into existing setups, while its low-cost oscillator keeps advanced imaging within reach for most researchers.
With the VINCI, researchers gain the ability to explore deeper tissue imaging and more complex biological systems, enabling earlier diagnosis of conditions like tumors, vascular diseases, and neurodegenerative disorders. The flexibility to combine 920 nm and 1064 nm wavelengths further enhances imaging capabilities, making simultaneous multicolour imaging both practical and precise.
The VINCI femtosecond laser transforms the limitations of traditional Ti:Sa systems into new opportunities for scientific discovery. By upgrading to this innovative light source, researchers can push the boundaries of their imaging capabilities, achieving unprecedented depth, resolution, and fluorescence intensity. Book a meeting with Michel, Product line Manager, today to learn how the VINCI can enhance your two-photon microscopy and empower your research.